Application
Western blot analysis of UROS Antibody (N-term) in K562 cell line lysates (35ug/lane). UROS (arrow) was detected using the purified Pab.Application
Western blot analysis of UROS Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). UROS (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with UROS Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
UROS Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of UROS (arrow) using rabbit polyclonal UROS Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UROS gene.| Product Name | UROS Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UROS; Uroporphyrinogen-III synthase; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | UROS Uroporphyrinogen-III synthase Hydroxymethylbilane hydrolyase [cyclizing] Uroporphyrinogen-III cosynthase |
|---|---|
| Molecular Weight(MW) | 28628 Da |
| Function | Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of porphyrins. Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as methionine synthesis (vitamin B12) or oxygen transport (heme) |
| Tissue Specificity | Ubiquitous. |
| Entrez Gene | 7390 |
|---|
Application
Western blot analysis of UROS Antibody (N-term) in K562 cell line lysates (35ug/lane). UROS (arrow) was detected using the purified Pab.
Application
Western blot analysis of UROS Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). UROS (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with UROS Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
UROS Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of UROS (arrow) using rabbit polyclonal UROS Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UROS gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
