Application
USP10 Antibody (Center) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the USP10 antibody detected the USP10 protein (arrow).| Product Name | USP10 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | USP10; KIAA0190; Ubiquitin carboxyl-terminal hydrolase 10; Deubiquitinating enzyme 10; Ubiquitin thioesterase 10; Ubiquitin-specific-processing protease 10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0190 |
|---|---|
| Alternative Names | USP10 KIAA0190 Ubiquitin carboxyl-terminal hydrolase 10 Deubiquitinating enzyme 10 Ubiquitin thioesterase 10 Ubiquitin-specific-processing protease 10 |
| Molecular Weight(MW) | 87134 Da |
| Function | Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34- containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Cytoplasm. Nucleus. Early endosome. Note=Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM |
| Entrez Gene | 9100 |
|---|---|
| Nucleotide Accession | NP_005144.2 |
Application
USP10 Antibody (Center) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the USP10 antibody detected the USP10 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
