Application
Western blot analysis of hUSP21-L241 in NCI-H460 cell line lysates (35ug/lane). USP21 (arrow) was detected using the purified Pab.| Product Name | USP21 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | USP23 |
|---|---|
| Alternative Names | USP21 USP23 Ubiquitin carboxyl-terminal hydrolase 21 Deubiquitinating enzyme 21 Ubiquitin thioesterase 21 Ubiquitin-specific-processing protease 21 |
| Molecular Weight(MW) | 62656 Da |
| Function | Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity) Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates |
| Tissue Specificity | Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 27005 |
|---|
Application
Western blot analysis of hUSP21-L241 in NCI-H460 cell line lysates (35ug/lane). USP21 (arrow) was detected using the purified Pab.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
