Application
The anti-USP28 Pab is used in Western blot to detect USP28 in Jurkat cell lysate.| Product Name | USP28 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | USP28; KIAA1515; Ubiquitin carboxyl-terminal hydrolase 28; Deubiquitinating enzyme 28; Ubiquitin thioesterase 28; Ubiquitin-specific-processing protease 28 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1515 |
|---|---|
| Alternative Names | USP28 KIAA1515 Ubiquitin carboxyl-terminal hydrolase 28 Deubiquitinating enzyme 28 Ubiquitin thioesterase 28 Ubiquitin-specific-processing protease 28 |
| Molecular Weight(MW) | 122491 Da |
| Function | Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C) In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus |
| Tissue Specificity | This USP28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119~149 amino acids from the N-terminal region of human USP28. |
| Cellular Localization | Nucleus, nucleoplasm. |
Application
The anti-USP28 Pab is used in Western blot to detect USP28 in Jurkat cell lysate.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
