Application
Western blot analysis of hUSP3-Y505 in K562, A375, NCI-H460 cell line lysates (35ug/lane). USP3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | USP3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | USP3; Ubiquitin carboxyl-terminal hydrolase 3; Deubiquitinating enzyme 3; Ubiquitin thioesterase 3; Ubiquitin-specific-processing protease 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | USP3 Ubiquitin carboxyl-terminal hydrolase 3 Deubiquitinating enzyme 3 Ubiquitin thioesterase 3 Ubiquitin-specific-processing protease 3 |
|---|---|
| Molecular Weight(MW) | 58897 Da |
| Function | Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin |
| Tissue Specificity | Expressed in all tissues examined, with strongest expression in pancreas |
| Cellular Localization | Nucleus. Note=Localizes preferentially with monoubiquitinated H2A to chromatin |
| Entrez Gene | 9960 |
|---|
Application
Western blot analysis of hUSP3-Y505 in K562, A375, NCI-H460 cell line lysates (35ug/lane). USP3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
