Application
USP6 Antibody (E978) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the USP6 antibody detected the USP6 protein (arrow).Application
USP6 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | USP6 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HRP1, TRE2 |
|---|---|
| Alternative Names | USP6 HRP1 TRE2 Ubiquitin carboxyl-terminal hydrolase 6 Deubiquitinating enzyme 6 Proto-oncogene TRE-2 Ubiquitin thioesterase 6 Ubiquitin-specific-processing protease 6 |
| Molecular Weight(MW) | 158658 Da |
| Function | Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6- dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation |
| Tissue Specificity | Testis specific. Expressed in various cancer cell lines |
| Cellular Localization | Cell membrane. Cytoplasm. Endosome. Note=Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton |
| Entrez Gene | 9098 |
|---|
Application
USP6 Antibody (E978) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the USP6 antibody detected the USP6 protein (arrow).
Application
USP6 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
