Application
Fluorescent image of U251 cells stained with UVRAG (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1850b UVRAG (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). UVRAG immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Application
Immunoprecipitation and western blot analysis of anti-UVRAG Pab in 293T cells. UVRAG is immunoprecipitated (Lane 2) and detected in 293T cell transiently transfected with mouse UVRAG (Lane 1). Detection of endogenous UVRAG is shown in 293T cells (Lane 3) but is reduced by UVRAG siRNA transfection (Lane 4). Data courtesy of Dr. Hong-Gang Wang, Moffitt Cancer Center, Tampa, FL.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | UVRAG Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IF,WB,IHC |
| IF:1:200 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | UVRAG UV radiation resistance-associated gene protein p63 |
|---|---|
| Molecular Weight(MW) | 78151 Da |
| Tissue Specificity | Highly expressed in brain, lung, kidney and liver |
| Cellular Localization | Late endosome. Lysosome. Early endosome. Note=Colocalizes with RAB9-positive compartments involved in retrograde transport from late endosomes to trans-Golgi network Colocalization with early endosomes is only partial |
| Entrez Gene | 7405 |
|---|
Application
Fluorescent image of U251 cells stained with UVRAG (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1850b UVRAG (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). UVRAG immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application
Immunoprecipitation and western blot analysis of anti-UVRAG Pab in 293T cells. UVRAG is immunoprecipitated (Lane 2) and detected in 293T cell transiently transfected with mouse UVRAG (Lane 1). Detection of endogenous UVRAG is shown in 293T cells (Lane 3) but is reduced by UVRAG siRNA transfection (Lane 4). Data courtesy of Dr. Hong-Gang Wang, Moffitt Cancer Center, Tampa, FL.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | IF:1:200 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
