Application
Western blot analysis of VAMP5 Antibody (N-term) in MDA-MB435 cell line lysates (35ug/lane). VAMP5 (arrow) was detected using the purified Pab.Application
VAMP5 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VAMP5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
VAMP5 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | VAMP5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | VAMP5 Vesicle-associated membrane protein 5 Myobrevin |
|---|---|
| Molecular Weight(MW) | 12805 Da |
| Function | May participate in trafficking events that are associated with myogenesis, such as myoblast fusion and/or GLUT4 trafficking |
| Tissue Specificity | This VAMP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-40 amino acids from the N-terminal region of human VAMP5. |
| Cellular Localization | Cell membrane; Single-pass type IV membrane protein (Probable). Endomembrane system; Single-pass type IV membrane protein (Probable). Golgi apparatus, trans-Golgi network membrane; Single-pass type IV membrane protein (Probable) Note=Associated with the plasma membrane as well as intracellular perinuclear and peripheral vesicular structures of myotubes Associated with the trans-Golgi, but not with the cis-Golgi apparatus (By similarity) |
| Entrez Gene | 10791 |
|---|
Application
Western blot analysis of VAMP5 Antibody (N-term) in MDA-MB435 cell line lysates (35ug/lane). VAMP5 (arrow) was detected using the purified Pab.
Application
VAMP5 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VAMP5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
VAMP5 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
