Application
VAMP7 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the VAMP7 antibody detected the VAMP7 protein (arrow).Application
VAMP7 Antibody (Center) (AP13865c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VAMP7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | VAMP7 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SYBL1 |
|---|---|
| Alternative Names | VAMP7 SYBL1 Vesicle-associated membrane protein 7 Synaptobrevin-like protein 1 Tetanus-insensitive VAMP |
| Molecular Weight(MW) | 24935 Da |
| Function | Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation |
| Tissue Specificity | Detected in all tissues tested. |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type IV membrane protein (By similarity) Golgi apparatus, trans-Golgi network membrane; Single-pass type IV membrane protein (By similarity). Late endosome membrane; Single- pass type IV membrane protein (By similarity). Lysosome membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein (By similarity) Cytoplasmic vesicle, phagosome membrane; Single-pass type IV membrane protein (By similarity). Cell junction, synapse, synaptosome (By similarity). Note=In immature neurons expression is localized in vesicular structures in axons and dendrites while in mature neurons it is localized to the somatodendritic region Colocalizes with LAMP1 in kidney cells. Localization to the endoplasmic reticulum membrane was observed in the intestine but not in liver or kidney (By similarity) |
| Entrez Gene | 6845 |
|---|---|
| Nucleotide Accession | NP_001138621.1 NP_005629.1 NP_001172112.1 |
Application
VAMP7 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the VAMP7 antibody detected the VAMP7 protein (arrow).
Application
VAMP7 Antibody (Center) (AP13865c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VAMP7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
