Application
A. Western blot analysis on Jurkat cell lysate using anti-Vav3 RabMAb (Cat. #AJ1811a).Application
B. Flow cytometric analysis of permeabilized Jurkat cells using anti-VAV3 pan RabMAb (red) (Cat. #AJ1811a) or a rabbit IgG (negative) (green).| Product Name | Vav3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1130Y |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500~1000 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 97776 Da |
|---|---|
| Function | Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis Its recruitment by phosphorylated EPHA2 is critical for EFNA1- induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)- mediated adhesion. Does not affect integrin beta-1 (ITGB1)- mediated adhesion (By similarity) |
| Tissue Specificity | Isoform 1 and isoform 3 are widely expressed; both are expressed at very low levels in skeletal muscle. In keratinocytes, isoform 1 is less abundant than isoform 3. Isoform 3 is detected at very low levels, if any, in adrenal gland, bone marrow, spleen, fetal brain and spinal chord; in these tissues, isoform 1 is readily detectable |
| Entrez Gene | 10451 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-Vav3 RabMAb (Cat. #AJ1811a).
Application
B. Flow cytometric analysis of permeabilized Jurkat cells using anti-VAV3 pan RabMAb (red) (Cat. #AJ1811a) or a rabbit IgG (negative) (green).| Application Notes | WB:1:500~1000 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
