Application
Western blot analysis of anti-CDH5 Pab in 293 cell line lysates (35ug/lane). CDH5(arrow) was detected using the purified Pab (1:60 dilution).Application
Flow cytometric analysis of 293 cells using VE Cadherin Antibody (CDH5) (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | VE Cadherin Antibody (CDH5) (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50-100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDH5 Cadherin-5 7B4 antigen Vascular endothelial cadherin CD_antigen=CD144 Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 87528 Da |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction |
| Tissue Specificity | Endothelial tissues and brain. |
| Cellular Localization | Cell junction. Cell membrane; Single-pass type I membrane protein (Probable). Note=Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions |
| Entrez Gene | 1003 |
|---|
Application
Western blot analysis of anti-CDH5 Pab in 293 cell line lysates (35ug/lane). CDH5(arrow) was detected using the purified Pab (1:60 dilution).
Application
Flow cytometric analysis of 293 cells using VE Cadherin Antibody (CDH5) (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50-100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
