Application
VILL Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the VILL antibody detected the VILL protein (arrow).Application
VILL Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VILL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of VILL Antibody (Center)(Cat#AP12085c) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
VILL Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | VILL Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VILL; Villin-like protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | VILL Villin-like protein |
|---|---|
| Molecular Weight(MW) | 95907 Da |
| Function | Possible tumor suppressor. |
| Tissue Specificity | Ubiquitously expressed in 16 tissues examined. |
| Entrez Gene | 50853 |
|---|---|
| Nucleotide Accession | NP_056957.3 |
Application
VILL Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the VILL antibody detected the VILL protein (arrow).
Application
VILL Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VILL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of VILL Antibody (Center)(Cat#AP12085c) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
VILL Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
