Application
Western blot analysis of VIME Antibody in A375 cell line lysates (35ug/lane). VIME (arrow) was detected using the purified Pab.Application
VIME Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VIME Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
VIME Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | VIME Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | VIM Vimentin |
|---|---|
| Molecular Weight(MW) | 53652 Da |
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally |
| Tissue Specificity | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 7431 |
|---|
Application
Western blot analysis of VIME Antibody in A375 cell line lysates (35ug/lane). VIME (arrow) was detected using the purified Pab.
Application
VIME Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VIME Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
VIME Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
