Application
A. Western blot analysis on 1) AP treated and 2) untreated HeLa + Calyculin A cell lysate using anti-Vimentin (pS82) RabMab (Cat. #AJ1815e).| Product Name | Vimentin Antibody Phospho (pS82) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S82 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1071Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:10000~50000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 53652 Da |
|---|---|
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally |
| Tissue Specificity | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 7431 |
|---|
Application
A. Western blot analysis on 1) AP treated and 2) untreated HeLa + Calyculin A cell lysate using anti-Vimentin (pS82) RabMab (Cat. #AJ1815e).| Application Notes | WB:1:10000~50000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
