Application
Western blot analysis of anti-VINC Antibody (N-term) in K562 cell line lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.Application
Western blot analysis of anti-VINC Antibody (N-term) in mouse lung tissue lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with VINC antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | VINC Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | VCL Vinculin Metavinculin |
|---|---|
| Molecular Weight(MW) | 123799 Da |
| Function | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion |
| Tissue Specificity | Metavinculin is muscle-specific. |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Note=Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity). Co-localizes with LIMD1 in the focal adhesions |
| Entrez Gene | 7414 |
|---|
Application
Western blot analysis of anti-VINC Antibody (N-term) in K562 cell line lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-VINC Antibody (N-term) in mouse lung tissue lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with VINC antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
