Application
VPS25 Antibody (C-term) western blot analysis in kidney cell line lysates (35ug/lane).This demonstrates the VPS25 antibody detected the VPS25 protein (arrow).| Product Name | VPS25 (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VPS25; DERP9; EAP20; Vacuolar protein-sorting-associated protein 25; Dermal papilla-derived protein 9; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DERP9 EAP20 |
|---|---|
| Alternative Names | VPS25 DERP9 EAP20 Vacuolar protein-sorting-associated protein 25 Dermal papilla-derived protein 9 ELL-associated protein of 20 kDa ESCRT-II complex subunit VPS25 |
| Molecular Weight(MW) | 20748 Da |
| Function | Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses |
| Tissue Specificity | Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung |
| Cellular Localization | Cytoplasm. Endosome membrane. Nucleus, nucleoplasm. Note=Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6 |
| Entrez Gene | 84313 |
|---|---|
| Nucleotide Accession | NP_115729.1 |
Application
VPS25 Antibody (C-term) western blot analysis in kidney cell line lysates (35ug/lane).This demonstrates the VPS25 antibody detected the VPS25 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
