Application
VPS4B Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the VPS4B antibody detected the VPS4B protein (arrow).| Product Name | VPS4B Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SKD1, VPS42 |
|---|---|
| Alternative Names | VPS4B SKD1 VPS42 Vacuolar protein sorting-associated protein 4B Cell migration-inducing gene 1 protein Suppressor of K(+) transport growth defect 1 |
| Molecular Weight(MW) | 49302 Da |
| Function | Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses) |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein (Probable). Note=Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells |
| Entrez Gene | 9525 |
|---|---|
| Nucleotide Accession | NP_004860.2 |
Application
VPS4B Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the VPS4B antibody detected the VPS4B protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
