Application
Western blot analysis of VPS53 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab.Application
Western blot analysis of VPS53 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with VPS53 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of MDA-MB468 cells using VPS53 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | VPS53 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | VPS53 Vacuolar protein sorting-associated protein 53 homolog |
|---|---|
| Molecular Weight(MW) | 79653 Da |
| Function | May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD |
| Tissue Specificity | This VPS53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118~148 amino acids from the N-terminal region of human VPS53. |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein |
| Entrez Gene | 55275 |
|---|
Application
Western blot analysis of VPS53 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab.
Application
Western blot analysis of VPS53 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with VPS53 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of MDA-MB468 cells using VPS53 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
