Application
The anti-VRK1 Pab is used in Western blot to detect VRK1 in mouse lung tissue lysate (Lane 1) and HL-60 cell lysate (Lane 2).Application
Western blot analysis of VRK1 (arrow) using rabbit polyclonal VRK1 Antibody (E381) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VRK1 gene.| Product Name | VRK1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VRK1; Serine/threonine-protein kinase VRK1; Vaccinia-related kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | VRK1 Serine/threonine-protein kinase VRK1 Vaccinia-related kinase 1 |
|---|---|
| Molecular Weight(MW) | 45476 Da |
| Function | Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr- 18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3 Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm |
| Tissue Specificity | Widely expressed. Highly expressed in fetal liver, testis and thymus |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle (By similarity). Note=Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis |
| Entrez Gene | 7443 |
|---|
Application
The anti-VRK1 Pab is used in Western blot to detect VRK1 in mouse lung tissue lysate (Lane 1) and HL-60 cell lysate (Lane 2).
Application
Western blot analysis of VRK1 (arrow) using rabbit polyclonal VRK1 Antibody (E381) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VRK1 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
