Application
VRK2 Antibody (M1) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the VRK2 antibody detected the VRK2 protein (arrow).Application
Western blot analysis of VRK2 (arrow) using rabbit polyclonal VRK2 Antibody (M1) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VRK2 gene.| Product Name | VRK2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VRK2; Serine/threonine-protein kinase VRK2; Vaccinia-related kinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | VRK2 Serine/threonine-protein kinase VRK2 Vaccinia-related kinase 2 |
|---|---|
| Molecular Weight(MW) | 58141 Da |
| Function | Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant |
| Tissue Specificity | Isoform 1 and isoform 2 are expressed in various tumor cell lines. Expression of isoform 1 inversely correlates with ERBB2 in breast carcinomas (at protein level) Widely expressed. Highly expressed in fetal liver, skeletal muscle, pancreas, heart, peripheral blood leukocytes and testis |
| Cellular Localization | Isoform 1: Cytoplasm. Endoplasmic reticulum membrane; Single-pass type IV membrane protein (Potential) Mitochondrion membrane; Single-pass type IV membrane protein (Potential) |
Application
VRK2 Antibody (M1) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the VRK2 antibody detected the VRK2 protein (arrow).
Application
Western blot analysis of VRK2 (arrow) using rabbit polyclonal VRK2 Antibody (M1) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VRK2 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
