Application
Western blot analysis of VTI1A Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). VTI1A (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma with VTI1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of NCI-H292 cells using VTI1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of VTI1A (arrow) using rabbit polyclonal VTI1A Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VTI1A gene.| Product Name | VTI1A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VTI1A; Vesicle transport through interaction with t-SNAREs homolog 1A; Vesicle transport v-SNARE protein Vti1-like 2; Vti1-rp2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | VTI1A Vesicle transport through interaction with t-SNAREs homolog 1A Vesicle transport v-SNARE protein Vti1-like 2 Vti1-rp2 |
|---|---|
| Molecular Weight(MW) | 25218 Da |
| Function | V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non- conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence |
| Tissue Specificity | This VTI1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147~177 amino acids from the C-terminal region of human VTI1A. |
| Cellular Localization | Cytoplasmic vesicle. Golgi apparatus membrane; Single-pass type IV membrane protein (By similarity) |
| Entrez Gene | 143187 |
|---|
Application
Western blot analysis of VTI1A Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). VTI1A (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with VTI1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of NCI-H292 cells using VTI1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of VTI1A (arrow) using rabbit polyclonal VTI1A Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VTI1A gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
