Application
Western blot analysis of WDR82 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). WDR82 (arrow) was detected using the purified Pab.Application
WDR82 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR82 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
WDR82 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | WDR82 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WDR82; WDR82A; WD repeat-containing protein 82; Swd2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TMEM113 WDR82A |
|---|---|
| Alternative Names | WDR82 WDR82A WD repeat-containing protein 82 Swd2 |
| Molecular Weight(MW) | 35079 Da |
| Function | Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase |
| Tissue Specificity | This WDR82 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~31 amino acids from the N-terminal region of human WDR82. |
| Cellular Localization | Nucleus. Note=Associates with chromatin. |
| Entrez Gene | 80335 |
|---|
Application
Western blot analysis of WDR82 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). WDR82 (arrow) was detected using the purified Pab.
Application
WDR82 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR82 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
WDR82 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
