Application
Western blot analysis of anti-WDYHV1 Antibody (Center) Pab in mouse heart tissue lysates (35ug/lane). WDYHV1(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with C8orf32 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | WDYHV1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WDYHV1; C8orf32; NTAQ1; Protein N-terminal glutamine amidohydrolase; Protein NH2-terminal glutamine deamidase; WDYHV motif-containing protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C8orf32 NTAQ1 |
|---|---|
| Alternative Names | WDYHV1 C8orf32 NTAQ1 Protein N-terminal glutamine amidohydrolase Protein NH2-terminal glutamine deamidase WDYHV motif-containing protein 1 |
| Molecular Weight(MW) | 23680 Da |
| Function | Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N- terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C- terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine (By similarity) |
| Tissue Specificity | This WDYHV1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114~143 amino acids from the Center region of human WDYHV1. |
| Cellular Localization | Cytoplasm, cytosol (By similarity). Nucleus (By similarity) |
| Entrez Gene | 55093 |
|---|
Application
Western blot analysis of anti-WDYHV1 Antibody (Center) Pab in mouse heart tissue lysates (35ug/lane). WDYHV1(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with C8orf32 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
