Application
WIBG Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the WIBG antibody detected the WIBG protein (arrow).Application
WIBG antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WIBG antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | WIBG Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WIBG; PYM; Partner of Y14 and mago; Protein wibg homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PYM |
|---|---|
| Alternative Names | WIBG PYM Partner of Y14 and mago Protein wibg homolog |
| Molecular Weight(MW) | 22656 Da |
| Function | Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post- transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC |
| Tissue Specificity | This WIBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human WIBG. |
| Cellular Localization | Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Shuttles between the nucleus and the cytoplasm Nuclear export is mediated by XPO1/CRM1 |
| Entrez Gene | 84305 |
|---|---|
| Nucleotide Accession | NP_115721.1 |
Application
WIBG Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the WIBG antibody detected the WIBG protein (arrow).
Application
WIBG antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WIBG antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
