Application
PRKWNK3 Antibody western blot analysis in UM cell line lysates (35ug/lane).This demonstrates the PRKWNK3 antibody detected the PRKWNK3 protein (arrow).Application
WNK3(PRKWNK3) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WNK3(PRKWNK3) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | WNK3 (PRKWNK3) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein kinase WNK3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1566 PRKWNK3 |
|---|---|
| Alternative Names | Protein kinase WNK3 |
| Molecular Weight(MW) | 198416 Da |
| Function | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Phosphorylates WNK4 Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner |
| Tissue Specificity | Expressed in brain, lung, kidney, liver and pancreas, and in fetal tissues including placenta, fetal brain, lung and kidney. Very low levels of expression were also detected in fetal heart, thymus, liver and spleen. Isoform 1 is brain- specific. Isoform 3 is kidney-specific |
| Cellular Localization | Cytoplasm. |
Application
PRKWNK3 Antibody western blot analysis in UM cell line lysates (35ug/lane).This demonstrates the PRKWNK3 antibody detected the PRKWNK3 protein (arrow).
Application
WNK3(PRKWNK3) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WNK3(PRKWNK3) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.10% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
