Application
Figure 1: Western blot analysis using anti-WNT1 monoclonal antibody against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.Application
Figure 2: Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using anti-WNT1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Application
Figure 4: Flow cytometric analysis of Hela cells using anti-WNT1 mAb (green) and negative control (purple).| Product Name | WNT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | INT1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10C8 |
| Host Species | Mouse |
| Tested Applications | WBIFFC |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | INT1 |
|---|---|
| Alternative Names | INT1 |
| Molecular Weight(MW) | 40982 Da |
| Function | Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters |
| Tissue Specificity | Purified recombinant fragment of WNT1 expressed in E. Coli. |
| Cellular Localization | Secreted, extracellular space, extracellular matrix |
| Entrez Gene | 7471 |
|---|---|
| Nucleotide Accession | NP_005421.1 |
Application
Figure 1: Western blot analysis using anti-WNT1 monoclonal antibody against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.
Application
Figure 2: Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using anti-WNT1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 4: Flow cytometric analysis of Hela cells using anti-WNT1 mAb (green) and negative control (purple).| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of WNT1 expressed in E. Coli. |
