Application
A. Western blot analysis on HeLa cell lysate using anti-WSTF RabMAb (Cat. #AJ1821a).Application
B. Immunohistochemical staining of paraffin-embedded human lymphoma using anti-WTSF RabMAb (Cat. #AJ1821a).| Product Name | WSTF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BAZ1B, WBSC10, WBSCR10, WBSCR9, WSTF, Tyrosine-protein kinase BAZ1B, Bromodomain adjacent to zinc finger domain protein 1B;Williams-Beuren syndrome chromosomal region 9 protein;Williams syndrome transcription factor |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1704Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | WBSC10 WBSCR10 WBSCR9 WSTF |
|---|---|
| Alternative Names | BAZ1B WBSC10 WBSCR10 WBSCR9 WSTF Tyrosine-protein kinase BAZ1B Bromodomain adjacent to zinc finger domain protein 1B Williams-Beuren syndrome chromosomal region 9 protein Williams syndrome transcription factor |
| Molecular Weight(MW) | 170903 Da |
| Function | Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association |
| Tissue Specificity | Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary |
| Cellular Localization | Nucleus. Note=Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA |
| Entrez Gene | 9031 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-WSTF RabMAb (Cat. #AJ1821a).
Application
B. Immunohistochemical staining of paraffin-embedded human lymphoma using anti-WTSF RabMAb (Cat. #AJ1821a).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
