Application
WT1 Antibody (Center E361) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the WT1 antibody detected the WT1 protein (arrow).Application
WT1 Antibody (Center E361) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WT1 Antibody (Center E361) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of WT1 Antibody (Center E361) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
WT1 Antibody (Center E361) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | WT1 Antibody (Center E361) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WT1; Wilms tumor protein; WT33 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | WT1 Wilms tumor protein WT33 |
|---|---|
| Molecular Weight(MW) | 49188 Da |
| Function | Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA |
| Tissue Specificity | Expressed in the kidney and a subset of hematopoietic cells |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Cytoplasm (By similarity). Note=Shuttles between nucleus and cytoplasm (By similarity) Isoform 4: Nucleus, nucleoplasm. |
Application
WT1 Antibody (Center E361) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the WT1 antibody detected the WT1 protein (arrow).
Application
WT1 Antibody (Center E361) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WT1 Antibody (Center E361) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of WT1 Antibody (Center E361) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
WT1 Antibody (Center E361) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
