Application
WRN Antibody (Center T802) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the WRN antibody detected the WRN protein (arrow).Application
WRN Antibody (Center T802) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | WRN Antibody (Center T802) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WRN; RECQ3; RECQL2; Werner syndrome ATP-dependent helicase; DNA helicase, RecQ-like type 3; Exonuclease WRN; RecQ protein-like 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RECQ3 RECQL2 |
|---|---|
| Alternative Names | WRN RECQ3 RECQL2 Werner syndrome ATP-dependent helicase DNA helicase RecQ-like type 3 Exonuclease WRN RecQ protein-like 2 |
| Molecular Weight(MW) | 162461 Da |
| Function | Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair Alleviates stalling of DNA polymerases at the site of DNA lesions Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity) |
| Tissue Specificity | This WRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 794-825 amino acids from the Central region of human WRN. |
| Cellular Localization | Nucleus, nucleolus. Nucleus. |
| Entrez Gene | 7486 |
|---|---|
| Nucleotide Accession | NP_000544.2 |
Application
WRN Antibody (Center T802) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the WRN antibody detected the WRN protein (arrow).
Application
WRN Antibody (Center T802) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
