Application
XPO5 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the XPO5 antibody detected the XPO5 protein (arrow).| Product Name | XPO5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XPO5; KIAA1291; RANBP21; Exportin-5; Ran-binding protein 21 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1291 RANBP21 |
|---|---|
| Alternative Names | XPO5 KIAA1291 RANBP21 Exportin-5 Ran-binding protein 21 |
| Molecular Weight(MW) | 136311 Da |
| Function | Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner |
| Tissue Specificity | Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas |
| Cellular Localization | Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm |
| Entrez Gene | 57510 |
|---|---|
| Nucleotide Accession | NP_065801.1 |
Application
XPO5 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the XPO5 antibody detected the XPO5 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
