Application
XRCC1 Antibody (Center) western blot analysis in A375 cell line lysates (15ug/lane).This demonstrates the XRCC1 antibody detected the XRCC1 protein (arrow).Application
XRCC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
XRCC1 Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of XRCC1 Antibody (Center) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | XRCC1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XRCC1; DNA repair protein XRCC1; X-ray repair cross-complementing protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | XRCC1 DNA repair protein XRCC1 X-ray repair cross-complementing protein 1 |
|---|---|
| Molecular Weight(MW) | 69477 Da |
| Function | Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents |
| Tissue Specificity | This XRCC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-443 amino acids from the Central region of human XRCC1. |
| Cellular Localization | Nucleus. Note=Accumulates at sites of DNA damage |
| Entrez Gene | 7515 |
|---|---|
| Nucleotide Accession | NP_006288.2 |
Application
XRCC1 Antibody (Center) western blot analysis in A375 cell line lysates (15ug/lane).This demonstrates the XRCC1 antibody detected the XRCC1 protein (arrow).
Application
XRCC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
XRCC1 Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of XRCC1 Antibody (Center) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
