Application
XYLT1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the XYLT1 antibody detected the XYLT1 protein (arrow).Application
XYLT1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | XYLT1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XYLT1; XT1; Xylosyltransferase 1; Peptide O-xylosyltransferase 1; Xylosyltransferase I |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | XT1 |
|---|---|
| Alternative Names | XYLT1 XT1 Xylosyltransferase 1 Peptide O-xylosyltransferase 1 Xylosyltransferase I |
| Molecular Weight(MW) | 107569 Da |
| Function | Catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes |
| Tissue Specificity | Widely expressed. Expressed at higher level in placenta, kidney and pancreas. Weakly expressed in skeletal muscle |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein (By similarity). Golgi apparatus membrane; Single-pass type II membrane protein (By similarity) Secreted (Probable). Note=Some fraction is also found in the extracellular space together with chondroitin sulfate proteoglycans, suggesting that it can be secreted |
| Entrez Gene | 64131 |
|---|---|
| Nucleotide Accession | NP_071449.1 |
Application
XYLT1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the XYLT1 antibody detected the XYLT1 protein (arrow).
Application
XYLT1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
