Application
YI016 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YI016 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
YI016 Antibody (C-term) western blot analysis in K562,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the YI016 antibody detected the YI016 protein (arrow).Application
Confocal immunofluorescent analysis of YI016 Antibody (C-term)(Cat#AP11654b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
YI016 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | YI016 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Putative tubulin beta chain-like protein ENSP00000290377 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBIFFC |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Putative tubulin beta chain-like protein ENSP00000290377 |
|---|---|
| Molecular Weight(MW) | 41775 Da |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity) |
| Tissue Specificity | This YI016 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human YI016. |
| Cellular Localization | Cytoplasm, cytoskeleton (By similarity). |
Application
YI016 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YI016 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
YI016 Antibody (C-term) western blot analysis in K562,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the YI016 antibody detected the YI016 protein (arrow).
Application
Confocal immunofluorescent analysis of YI016 Antibody (C-term)(Cat#AP11654b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
YI016 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
