Application
YOD1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the YOD1 antibody detected the YOD1 protein (arrow).Application
YOD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YOD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of YOD1 Antibody (C-term)(Cat#AP11304b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | YOD1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YOD1; DUBA8; HIN7; OTUD2; Ubiquitin thioesterase OTU1; DUBA-8; HIV-1-induced protease 7; OTU domain-containing protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DUBA8 HIN7 OTUD2 |
|---|---|
| Alternative Names | YOD1 DUBA8 HIN7 OTUD2 Ubiquitin thioesterase OTU1 DUBA-8 HIV-1-induced protease 7 OTU domain-containing protein 2 |
| Molecular Weight(MW) | 38322 Da |
| Function | Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di- ubiquitin |
| Tissue Specificity | This YOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-347 amino acids from the C-terminal region of human YOD1. |
| Entrez Gene | 55432 |
|---|---|
| Nucleotide Accession | NP_061036.3 |
Application
YOD1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the YOD1 antibody detected the YOD1 protein (arrow).
Application
YOD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YOD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of YOD1 Antibody (C-term)(Cat#AP11304b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
