Application
Western blot analysis of YMEL1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). YMEL1 (arrow) was detected using the purified Pab.Application
Western blot analysis of YMEL1 Antibody (N-term) in CHO cell line lysates (35ug/lane). YMEL1 (arrow) was detected using the purified Pab.Application
YMEL1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YMEL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
YMEL1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | YMEL1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YME1L1; FTSH1; YME1L; ATP-dependent zinc metalloprotease YME1L1; ATP-dependent metalloprotease FtsH1; Meg-4; Presenilin-associated metalloprotease; YME1-like protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FTSH1 YME1L |
|---|---|
| Alternative Names | YME1L1 FTSH1 YME1L ATP-dependent zinc metalloprotease YME1L1 ATP-dependent metalloprotease FtsH1 Meg-4 Presenilin-associated metalloprotease YME1-like protein 1 |
| Molecular Weight(MW) | 86455 Da |
| Function | Putative ATP-dependent protease which plays a role in mitochondrial protein metabolism. Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. Requires to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1). Seems to act in the processing of OPA1 |
| Tissue Specificity | High expression in cardiac and skeletal muscle mitochondria |
| Cellular Localization | Mitochondrion inner membrane. |
| Entrez Gene | 10730 |
|---|
Application
Western blot analysis of YMEL1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). YMEL1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of YMEL1 Antibody (N-term) in CHO cell line lysates (35ug/lane). YMEL1 (arrow) was detected using the purified Pab.
Application
YMEL1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YMEL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
YMEL1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
