Application
A. Western blot analysis of HeLa cell lysate probed with anti-14-3-3 b/a RabMab (Cat. #AJ1001b).Application
B. Immunohistochemical analysis of human skin using anti-14-3-3 b/a RabMab (Cat. #AJ1001b).Application
C. Flow cytometric analysis of permeabilized HeLa cells using anti-14-3-3 b/a RabMAb (red) (Cat. #AJ1001b) or a rabbit IgG (negative) (green).| Product Name | YWHAB Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YWHAB, 14-3-3 protein beta/alpha, N-terminally processed, Protein 1054 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E58 |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | YWHAB 14-3-3 protein beta/alpha N-terminally processed Protein 1054 |
|---|---|
| Molecular Weight(MW) | 28082 Da |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2 |
| Tissue Specificity | A synthetic peptide corresponding to residues near N-terminus of human 14-3-3 beta/alpha was used as immunogen. Predicted to cross-react with sheep and bovine, based on sequence homology. |
| Cellular Localization | Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 7529 |
|---|
Application
A. Western blot analysis of HeLa cell lysate probed with anti-14-3-3 b/a RabMab (Cat. #AJ1001b).
Application
B. Immunohistochemical analysis of human skin using anti-14-3-3 b/a RabMab (Cat. #AJ1001b).
Application
C. Flow cytometric analysis of permeabilized HeLa cells using anti-14-3-3 b/a RabMAb (red) (Cat. #AJ1001b) or a rabbit IgG (negative) (green).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
