Application
YWHAE Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YWHAE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
YWHAE Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of YWHAE Antibody (C-term)(Cat#AP10671b) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
YWHAE Antibody (C-term) western blot analysis in A375,HL-60,Ramos cell line and mouse cerebellum tissue lysates (35ug/lane).This demonstrates the YWHAE antibody detected the YWHAE protein (arrow).| Product Name | YWHAE Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YWHAE; 14-3-3 protein epsilon |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCFCIFWB |
| IHC:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | YWHAE 14-3-3 protein epsilon |
|---|---|
| Molecular Weight(MW) | 29174 Da |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner |
| Tissue Specificity | This YWHAE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-259 amino acids from the C-terminal region of human YWHAE. |
| Cellular Localization | Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
Application
YWHAE Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YWHAE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
YWHAE Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of YWHAE Antibody (C-term)(Cat#AP10671b) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
YWHAE Antibody (C-term) western blot analysis in A375,HL-60,Ramos cell line and mouse cerebellum tissue lysates (35ug/lane).This demonstrates the YWHAE antibody detected the YWHAE protein (arrow).| Application Notes | IHC:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
