Application
Western blot analysis of YWHAZ Antibody (Center) in MDA-MB231, A2058 cell line and mouse cerebellum, brain tissue lysates (35ug/lane). YWHAZ (arrow) was detected using the purified Pab.Application
YWHAZ Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YWHAZ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
YWHAZ Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | YWHAZ Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | YWHAZ 14-3-3 protein zeta/delta Protein kinase C inhibitor protein 1 |
|---|---|
| Molecular Weight(MW) | 27745 Da |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner |
| Tissue Specificity | This YWHAZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72~102 amino acids from the Center region of human YWHAZ. |
| Cellular Localization | Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes |
| Entrez Gene | 7534 |
|---|
Application
Western blot analysis of YWHAZ Antibody (Center) in MDA-MB231, A2058 cell line and mouse cerebellum, brain tissue lysates (35ug/lane). YWHAZ (arrow) was detected using the purified Pab.
Application
YWHAZ Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YWHAZ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
YWHAZ Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
