Application
Western blot analysis of YWHAQ Antibody (N-term) in MDA-MB231,Y79 cell line and mouse brain tissue lysates (35ug/lane). YWHAQ (arrow) was detected using the purified Pab.(2ug/ml)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with YWHAQ Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of MDA-MB468 cells using YWHAQ Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | YWHAQ Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | YWHAQ 14-3-3 protein theta 14-3-3 protein T-cell 14-3-3 protein tau Protein HS1 |
|---|---|
| Molecular Weight(MW) | 27764 Da |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner Negatively regulates the kinase activity of PDPK1 |
| Tissue Specificity | Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement |
| Cellular Localization | Cytoplasm. Note=In neurons, axonally transported to the nerve terminals |
| Entrez Gene | 10971 |
|---|
Application
Western blot analysis of YWHAQ Antibody (N-term) in MDA-MB231,Y79 cell line and mouse brain tissue lysates (35ug/lane). YWHAQ (arrow) was detected using the purified Pab.(2ug/ml)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with YWHAQ Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of MDA-MB468 cells using YWHAQ Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
