Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with 14-3-3 protein zeta/delta antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of hYWHAZ-D231 in mouse brain tissue and MCF-7 cell line lysates (35ug/lane). YWHAZ (arrow) was detected using the purified Pab.| Product Name | YWHAZ Antibody (D231) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | YWHAZ 14-3-3 protein zeta/delta Protein kinase C inhibitor protein 1 |
|---|---|
| Molecular Weight(MW) | 27745 Da |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner |
| Tissue Specificity | This YWHAZ-D231 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~245 amino acids from the C-terminal region of human 14-3-3 protein zeta/delta. |
| Cellular Localization | Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes |
| Entrez Gene | 7534 |
|---|
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with 14-3-3 protein zeta/delta antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of hYWHAZ-D231 in mouse brain tissue and MCF-7 cell line lysates (35ug/lane). YWHAZ (arrow) was detected using the purified Pab.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
