Application
Western blot analysis of ZAP128 Antibody (Center) in MCF-7 cell line lysates (35ug/lane).ZAP128 (arrow) was detected using the purified Pab.Application
ZAP128 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZAP128 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ZAP128 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZAP128 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACOT2; PTE2; PTE2A; Acyl-coenzyme A thioesterase 2, mitochondrial; Acyl-coenzyme A thioester hydrolase 2a; CTE-Ia; Long-chain acyl-CoA thioesterase 2; ZAP128 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PTE2 PTE2A |
|---|---|
| Alternative Names | ACOT2 PTE2 PTE2A Acyl-coenzyme A thioesterase 2 mitochondrial Acyl-coenzyme A thioester hydrolase 2a CTE-Ia Long-chain acyl-CoA thioesterase 2 ZAP128 |
| Molecular Weight(MW) | 53218 Da |
| Function | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH Displays high levels of activity on medium- and long chain acyl CoAs |
| Tissue Specificity | Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 10965 |
|---|---|
| Nucleotide Accession | NP_006812.3 |
Application
Western blot analysis of ZAP128 Antibody (Center) in MCF-7 cell line lysates (35ug/lane).ZAP128 (arrow) was detected using the purified Pab.
Application
ZAP128 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZAP128 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ZAP128 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
