Application
ZHX2 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ZHX2 antibody detected the ZHX2 protein (arrow).Application
Fluorescent confocal image of A549 cell stained with ZHX2 Antibody (N-term)(Cat#AP18752a).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZHX2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.| Product Name | ZHX2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AFR1, KIAA0854, RAF |
|---|---|
| Alternative Names | ZHX2 AFR1 KIAA0854 RAF Zinc fingers and homeoboxes protein 2 Alpha-fetoprotein regulator 1 Regulator of AFP Zinc finger and homeodomain protein 2 |
| Molecular Weight(MW) | 92307 Da |
| Function | Acts as a transcriptional repressor. Represses the promoter activity of the CDC25C gene stimulated by NFYA |
| Tissue Specificity | Ubiquitously expressed. Expressed in podocytes |
| Cellular Localization | Nucleus. |
| Entrez Gene | 22882 |
|---|---|
| Nucleotide Accession | NP_055758.1 |
Application
ZHX2 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ZHX2 antibody detected the ZHX2 protein (arrow).
Application
Fluorescent confocal image of A549 cell stained with ZHX2 Antibody (N-term)(Cat#AP18752a).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZHX2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
