Application
Western blot analysis of ZN473 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). ZN473 (arrow) was detected using the purified Pab.Application
ZN473 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZN473 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1141, ZFP100 |
|---|---|
| Alternative Names | ZNF473 KIAA1141 ZFP100 Zinc finger protein 473 Zinc finger protein 100 homolog |
| Molecular Weight(MW) | 100182 Da |
| Function | Involved in histone 3'-end pre-mRNA processing by associating with U7 snRNP and interacting with SLBP/pre-mRNA complex. Increases histone 3'-end pre-mRNA processing but has no effect on U7 snRNP levels, when overexpressed. Required for cell cycle progression from G1 to S phases |
| Tissue Specificity | This ZN473 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185~215 amino acids from the N-terminal region of human ZN473. |
| Cellular Localization | Nucleus. Note=Stable component of Cajal bodies (CBs). Colocalizes with SMN, coilin and U7 snRNA |
| Entrez Gene | 25888 |
|---|
Application
Western blot analysis of ZN473 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). ZN473 (arrow) was detected using the purified Pab.
Application
ZN473 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
