Application
Western blot analysis of ZNF281 (arrow) using rabbit polyclonal ZNF281 Antibody(Center) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ZNF281 gene (Lane 2) (Origene Technologies).| Product Name | ZNF281 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Zinc finger protein 281;ZNF281 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GZP1 ZBP99 |
|---|---|
| Alternative Names | Zinc finger protein 281 ZNF281 |
| Molecular Weight(MW) | 96915 Da |
| Function | Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G- rich box in the enhancer region of these genes |
| Tissue Specificity | This ZNF281 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 606~636 amino acids from the N-terminal region of human ZNF281. |
| Cellular Localization | Nucleus. |
Application
Western blot analysis of ZNF281 (arrow) using rabbit polyclonal ZNF281 Antibody(Center) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ZNF281 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
