Application
Western blot analysis of ZO1 Antibody (C-term) in K562 cell line lysates (35ug/lane). ZO1 (arrow) was detected using the purified Pab.Application
ZO1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZO1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ZO1 Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZO1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TJP1; ZO1; Tight junction protein ZO-1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | ELISAFCICC/IFIHCWB |
| WB:1:50~100 IHC |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ZO1 |
|---|---|
| Alternative Names | TJP1 ZO1 Tight junction protein ZO-1 Tight junction protein 1 Zona occludens protein 1 Zonula occludens protein 1 |
| Molecular Weight(MW) | 195459 Da |
| Function | The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells |
| Tissue Specificity | The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction Cell junction, gap junction. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells |
| Entrez Gene | 7082 |
|---|
Application
Western blot analysis of ZO1 Antibody (C-term) in K562 cell line lysates (35ug/lane). ZO1 (arrow) was detected using the purified Pab.
Application
ZO1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZO1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ZO1 Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
