Application
ZNRF1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ZNRF1 antibody detected the ZNRF1 protein (arrow).| Product Name | ZNRF1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZNRF1; NIN283; E3 ubiquitin-protein ligase ZNRF1; Nerve injury-induced gene 283 protein; Zinc/RING finger protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NIN283 |
|---|---|
| Alternative Names | ZNRF1 NIN283 E3 ubiquitin-protein ligase ZNRF1 Nerve injury-induced gene 283 protein Zinc/RING finger protein 1 |
| Molecular Weight(MW) | 23783 Da |
| Function | E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons (Probable) |
| Tissue Specificity | Expressed primarily in the nervous system, with expression higher in developing brain relative to adult Expressed at low levels in testis and thymus |
| Cellular Localization | Endosome. Lysosome. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein (Potential) Note=Associated with synaptic vesicle membranes in neurons |
| Entrez Gene | 84937 |
|---|---|
| Nucleotide Accession | NP_115644.1 |
Application
ZNRF1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ZNRF1 antibody detected the ZNRF1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
