Application
Western blot analysis on (A) fetal brain, (B) SH-SY5Y, (C) 293T and (D) SW480 cell lysates using anti-14-3-3E RabMAb (Cat. #AJ1001c).Application
Immunofluorescent staining of HeLa cells using anti-14-3-3E RabMAb (Cat. #AJ1001c).| Product Name | 14-3-3E Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3918 |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:20000~100000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 29174 Da |
|---|---|
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner |
| Tissue Specificity | A synthetic peptide corresponding to residues in human 14-3-3E was used as an immunogen. |
| Cellular Localization | Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 7531 |
|---|
Application
Western blot analysis on (A) fetal brain, (B) SH-SY5Y, (C) 293T and (D) SW480 cell lysates using anti-14-3-3E RabMAb (Cat. #AJ1001c).
Application
Immunofluorescent staining of HeLa cells using anti-14-3-3E RabMAb (Cat. #AJ1001c).| Application Notes | WB:1:20000~100000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
