Application
A. Western blot analysis on 293 cell lysate using anti-4E-BP1 RabMAb (Cat. #AJ1004a), dilution 1:10,000.Application
B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-4E-BP1 RabMAb (Cat. #AJ1004a).| Product Name | 4E-BP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y329 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:2000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EIF4EBP1 Eukaryotic translation initiation factor 4E-binding protein 1 Phosphorylated heat- and acid-stable protein regulated by insulin 1 |
|---|---|
| Molecular Weight(MW) | 12580 Da |
| Function | Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N-term of human4E-BP1 was used as immunogen. |
| Entrez Gene | 1978 |
|---|
Application
A. Western blot analysis on 293 cell lysate using anti-4E-BP1 RabMAb (Cat. #AJ1004a), dilution 1:10,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-4E-BP1 RabMAb (Cat. #AJ1004a).| Application Notes | WB:1:2000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
