Application
A. Western blot analysis on 293T cell lysates using anti-Phospho-4E-Bp1/2/3 (pT45) RabMAb (Cat. #AJ1004f), 1:10,000 dilution. Cells were either (A) untreated (B) treated with FBS.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-4E-BPE1 RabMAb (Cat. #AJ1004f).| Product Name | 4E-BP1/2/3 Antibody Phospho (pT45) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1 |
| Modification | p-T45 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2169Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EIF4EBP1 Eukaryotic translation initiation factor 4E-binding protein 1 Phosphorylated heat- and acid-stable protein regulated by insulin 1 |
|---|---|
| Molecular Weight(MW) | 12580 Da |
| Function | Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding threonine 45 was used as an immunogen. This antibody detects 4E-BP1/2/3 phosophorylated at threonine 45. |
| Entrez Gene | 1978 |
|---|
Application
A. Western blot analysis on 293T cell lysates using anti-Phospho-4E-Bp1/2/3 (pT45) RabMAb (Cat. #AJ1004f), 1:10,000 dilution. Cells were either (A) untreated (B) treated with FBS.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-4E-BPE1 RabMAb (Cat. #AJ1004f).| Application Notes | WB:1:500~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
