Application
Western blot analysis of AAK1 Antibody (N-term) in Ramos cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab.| Product Name | AAK1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AAK1; KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1048 |
|---|---|
| Alternative Names | AAK1 KIAA1048 AP2-associated protein kinase 1 Adaptor-associated kinase 1 |
| Molecular Weight(MW) | 103885 Da |
| Function | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity |
| Tissue Specificity | Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain |
| Cellular Localization | Cell membrane; Peripheral membrane protein (By similarity). Membrane, clathrin-coated pit (By similarity) Note=Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity) |
| Entrez Gene | 22848 |
|---|
Application
Western blot analysis of AAK1 Antibody (N-term) in Ramos cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
